IGG-BINDINGSPROTEIN

A recombinant DNA fragment (Z) coding for an immuno­globulin G binding domain related to staphylococcal protein A, characterized in that the methionine codon of said fragment has been replaced by a codon of another amino acid residue enabling expression of a methionine­free protein;a recombinant DNA...

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Bibliographic Details
Main Authors MOKS, TOMAS, UHLEN, MATHIAS, ABRAHMSEN, LARS, NILSSON, BJOERN
Format Patent
LanguageDanish
Published 14.06.1987
Edition4
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A recombinant DNA fragment (Z) coding for an immuno­globulin G binding domain related to staphylococcal protein A, characterized in that the methionine codon of said fragment has been replaced by a codon of another amino acid residue enabling expression of a methionine­free protein;a recombinant DNA sequence comprising at least two such fragments;a recombinant DMA sequence comprising such fragment preceded by a signal sequence and followed by a nucleotide sequence coding for the amino acid se­quence:Ala Gln His Asp Glu Ala;a recombinant DNA molecule comprising such recombinant DNA sequence and fused 3ʹ thereof at DNA level a pro­duction gene, said molecule having the ability to express a fused protein;a process for cleaving a fused protein expressed in a biological system by a such recombinant DNA molecule;a plasmid vector comprising said recombinant DNA molecule; anda bacterial cell harbouring such recombinant DNA molecule.
Bibliography:Application Number: DK19860006004