FREMGANGSMÅDE TIL SEPARATION AF ANTISTOFFER ELLER ANTISTOFFRAGMENTER UDEN EN FC-REGION SOM KAN BINDE TIL PROTEIN A
The invention discloses a method for separation of antibodies or antibody fragments, comprising the steps of: a) providing a feed comprising antibodies or antibody fragments having a VH3 region and being devoid of an Fc region capable of binding to Protein A; b) contacting the feed with a separation...
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Main Authors | , , , , |
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Format | Patent |
Language | Danish |
Published |
06.06.2023
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses a method for separation of antibodies or antibody fragments, comprising the steps of: a) providing a feed comprising antibodies or antibody fragments having a VH3 region and being devoid of an Fc region capable of binding to Protein A; b) contacting the feed with a separation resin having covalently coupled ligands, wherein the ligands comprise a polypeptide as defined by SEQ ID NO 1 and wherein the antibodies or antibody fragments bind to the separation resin; c) optionally washing the separation resin with a washing liquid; d) eluting the antibodies or antibody fragments from the separation resin with an elution liquid and recovering the antibodies or antibody fragments. |
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Bibliography: | Application Number: DK20723090T |