APPARAT TIL ENKELTMOLEKYLESEKVENTERING OG FREMGANGSMÅDE TIL SEKVENTERING AF NUKLEINSYREMOLEKYLER

• Main Authors: PAN, Chao-Chi, CIOU, Jian-Hao, CHIOU, Chung-Fan, TSAI, Ching-Wei, CHEN, Bor-Huah
• Format: Patent
• Language: Danish
• Published: 25.10.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS; OPTICS; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

An apparatus (100, 100a) suitable for single molecule sequencing. The apparatus (100, 100a) includes at least one nanowell (110), a plurality of nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e), and a plurality of types of nucleic acid fragments (130a, 130b, 130c, 130d, 130e). The nanowell (110) has an observation zone (112). The nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e) are disposed in or proximate to the observation zone (112). The nucleic acid fragments (130a, 130b, 130c, 130d, 130e) are immobilized to the nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e), respectively. At least one polymerase (140a, 140b, 140c, 140d) is disposed in the observation zone (112). A method of sequencing nucleic acid molecules using the above-mentioned apparatus is provided.