Ekspression af granulær-stivelse-hydrolyserende enzymer i Trichoderma og fremgangsmåde til fremstilling af glucose fra granulære stivelsessubstrater

• Main Authors: BALDWIN, TOBY, L, PEPSIN, MICHAEL, J, DUNN-COLEMAN, NIGEL, SHETTY, JAYARAMA, K, STROHM, BRUCE, A, BOWER, BENJAMIN S, LANTZ, SUZANNE, E, LANTERO, JR., ORESTE, CHOTANI, GOPAL, K
• Format: Patent
• Language: Danish
• Published: 02.07.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; ORGANIC MACROMOLECULAR COMPOUNDS; POLYSACCHARIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.