Fremgangsmåde til oplösning, oprensning og genfoldning af protein

• Main Authors: HALENBECK, ROBERT, F, TSANG, MICHAEL, HORA, MANINDER, S, DORIN, GLENN, J, MADANI, HASSAN, PATTISON, GREGORY, L, JOHNSON, GARY, V, JOHNSON, KIRK, RANA, RAJSHARAN, K, GUSTAFSON, MARK, E, BILD, GARY, S, ARVE, BO, H, CHEN, BAO-LU
• Format: Patent
• Language: Danish
• Published: 19.12.2005
• Edition: 6
• Subjects: CHEMISTRY; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES

Aqueous formulations of TFPI (tissue factor pathway inhibitor) include L-arginine and/or a solubilising agent selected from the group consisting of: an acetate ion; a sulfate ion; sodium chloride; a citrate ion; an isocitrate ion; glycine; glutamate; a succinate ion; histidine; imidazole; sodium dodecyl sulfate; or a charged polymer. The invention facilitates the solubilization, formulation, purification and refolding of TFPI.