Medlemmer af specifikt bindende par med en kemisk tilbundet kovalent inden for bindingsstedet, fremstilling af udvælgelse a

• Main Authors: JESPERS, LAURENT, STEPHANE, ANNE, THERESE, GREGORY, PAUL WINTER, SIMON, THOMAS, MARTIN, BONNERT, TIMOTHY, PETER
• Format: Patent
• Language: Danish
• Published: 02.04.2001
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

PCT No. PCT/GB94/01422 Sec. 371 Date Sep. 4, 1997 Sec. 102(e) Date Sep. 4, 1997 PCT Filed Jun. 30, 1994 PCT Pub. No. WO95/01438 PCT Pub. Date Jan. 12, 1995Repertoires of first specific binding pair (sbp) members wherein each first sbp member has a chemical moiety bound covalently at an amino acid residue within the binding site are made and may be displayed at the surface of an organism such as a bacteriophage. Methods of making such repertoires may involve the provision of a population of encoding nucleic acid molecules wherein a codon encoding a selectively or preferentially modifiable amino acid is introduced in the region encoding the binding site, for instance by mutation or gene construction. First sbp member (e.g. antibodies) wherein binding to second sbp member (e.g. antigen) is enhanced in or dependent on the presence of the chemical moiety in the binding site may be selected from the repertoires.