NUKLEINSÄURETYPISIERUNG DURCH POLYMERASEVERLÄNGERUNG VON OLIGONUKLEOTIDEN UNTER VERWENDUNG VON TERMINATOR-MISCHUNGEN

• Main Authors: GOELET, PHILIP, KNAPP, MICHAEL R, ANDERSON, STEPHEN
• Format: Patent
• Language: German
• Published: 29.12.2005
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

The present invention relates to a method of deforming the identity of one or more nucleotide bases of a plurality of specific positions in one or more nucleic acid molecules of interest. The method involves the provision of a single-stranded nucleic acid, which is contacted with hybridization printers to form duplexes that are contacted with a terminator reagent and undergo extension and affinity separation. This enables one or more nucleotide bases of a plurality of specific positions in one or more nucleic acid molecules of interest to be determined.