Nukleotidsequenz, die für ein Aussenmembran-Protein von Neisseria meningitidis kodiert und Verwendung dieses Proteins zur Herstellung von Impfstoffen

• Main Authors: COUZEAU RODRIGUEZ, EDELGIS, LA HABANA, CU, MORERA CORDOVA, VIVIAN, LA HABANA, CU, MUSACCHIO LASA, ALEXIS, LA HABANA, CU, MENENDEZ, EVELIN CABALLERO, BAUTA, LA HABANA, CU, NIETO, GERARDO GUILLEN, LA HABANA, CU, GONZALEZ BLANCO, SONIA, LA HABANA, CU, HERRERA MARTINEZ, LUIS S. CENTRO INGENIERIA, CUBANACAN. PLAYA HAVANA, CU, DEL VALLE ROSALES, JESUS AUGUSTO, LA VIBORA, LA HABANA, CU, SANTOS, BEATRIZ TAMARGO, SANCHEZ, BOYEROS, LA HABANA, CU, FERNANDEZ MASO, JULIO RAUL, LA HABANA, CU, CRUZ LEON, SILIAN, LA HABANA, CU, HOUSSEIN SOSA, MANUEL SELMAN, VEDADO, LA HABANA, CU, RODRIGUEZ, RICARDO SILVA, LA HABANA, CU, ALVAREZ ACOSTA, ANABEL, LA HABANA, CU, NOVOA PEREZ, LIDIA INES, LA HABANA, CU, GRILLO, JUAN MORALES, HABANA VIEJA, LA HABANA, CU
• Format: Patent
• Language: German
• Published: 27.11.1997
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The present invention is concerned with a method for the isolation of a nucleotide sequence which codes for a protein having a molecular weight of about 64 000 daltons, which is located on the outer membrane of N. meningitidis, as well as with the recombinant DNA obtained therefrom, which is used for the transformation of a host microorganism. The technical object pursued with the invention is the identification of a nucleotide sequence coding for a highly conserved and common protein for the majority of pathogenic Neisseria strains, the production of this protein with a high level of purity and in commercially useful amounts using the recombinant way, so that it can be used in diagnostic methods and vaccine preparations with a broad immunoprotection spectrum.