Cellulase, Verfahren zu deren Herstellung und deren Verwendung

A cellulase having the following properties: (1) an optimum pH of from 9.5 to 10.5 as measured using carboxymethylcellulose as a substrate; (2) a stable pH of from 6 to 11 as measured using carboxymethylcellulose as a substrate after treating at 30 DEG C and for 30 minutes; (3) an optimum temperatur...

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Bibliographic Details
Main Authors MASATSUJI, EIKO, SEKO, MASAHIKO, SAITO, KIYOSHI
Format Patent
LanguageGerman
Published 30.10.1997
Edition6
Subjects
ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES
APPARATUS THEREFOR
BEER
BIOCHEMISTRY
CANDLES
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DETERGENT COMPOSITIONS
DETERGENTS
ENZYMOLOGY
FATTY ACIDS THEREFROM
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PAPER-MAKING
PROCESSES USING MICROORGANISMS
PRODUCTION OF CELLULOSE
PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
RECOVERY OF GLYCEROL
REGENERATION OF PULPING LIQUORS
RESIN SOAPS
SOAP OR SOAP-MAKING
SPIRITS
TEXTILES
USE OF SINGLE SUBSTANCES AS DETERGENTS
VINEGAR
WINE
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Summary:A cellulase having the following properties: (1) an optimum pH of from 9.5 to 10.5 as measured using carboxymethylcellulose as a substrate; (2) a stable pH of from 6 to 11 as measured using carboxymethylcellulose as a substrate after treating at 30 DEG C and for 30 minutes; (3) an optimum temperature of about 55 DEG C as measured using carboxymethylcellulose as a substrate; (4) influence of a surfactant being such that residual activity is 90 % or more after treatment at 30 DEG C and pH of 9.0 for 2 hours in the presence of a sodium n-alkylbenzenesulfonate; (5) a molecular weight of 52,000 +/- 2,000 as measured by electrophoresis on SDS-polyacrylamide gel; and (6) an isoelectric point of 4.2 +/- 0.2 as measured by isoelectric electrophoresis. The cellulase is produced by Bacillus sp. SD402, its mutant strains or its genetic engineered strains. An aid for detergents and a paper treating agent, respectively, contain the cellulase as an effective ingredient.
Bibliography:Application Number: DE19916022687T