NEUER MEGAKARYOKYTISCHER KOLONIESTIMULIERENDER FAKTOR UND VERFAHREN ZUR HERSTELLUNG

According to this invention, a novel megakaryocytic colony stimulating factor is obtained by culturing a cell strain of human pulmonary large cell cancer, for example, PC-13 cell strain or human lung cancer cell strain MC-1 separated as monoclonal cells from PC-13 cell strain, recovering a supernata...

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Main Authors HAMAGUCHI, HIROYUKI, KOGANEI-SHI TOKYO 184, JP, KURIYA, SHINICHIRO NIPPON MEDICAL SCHOOL, TOKYO 113, JP, NOMURA, TAKEO NIPPON MEDICAL SCHOOL, TOKYO 113, JP, NAGAOKA, KOZO PHARMACEUTICAL RESEARCH-CENTER, KOHOKU-KU, YOKOHAMA-SHI KANAGAWA-KEN 222, JP, OGATA, KIYOYUKI NIPPON MEDICAL SCHOOL, BUNKYO-KU TOKYO 113, JP, NOJIRI, CHUHEI PHARMACEUTICAL RESEARCH-CENTER, KOHOKU-KU, YOKOHAMA-SHI KANAGAWA-KEN 222, JP, MAKABE, OSAMU PHARMACEUTICAL RESEARCH-CENTE, KOHOKU-KU, YOKOHAMA-SHI KANAGAWA-KEN 222, JP, MATSUNAGA, KEITA PHARMACEUTICAL RESEARCH-CENTE, KOHOKU-KU, YOKOHAMA-SHI KANAGAWA-KEN 222, JP
Format Patent
LanguageGerman
Published 02.10.1997
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:According to this invention, a novel megakaryocytic colony stimulating factor is obtained by culturing a cell strain of human pulmonary large cell cancer, for example, PC-13 cell strain or human lung cancer cell strain MC-1 separated as monoclonal cells from PC-13 cell strain, recovering a supernatant of the culture liquor, collecting from the supernatant a megakaryocytic colony stimulating factor (Meg-CSF) comprising a protein having the activity of forming a megakaryocytic colony from myeloid cells when applied to human or mouse myeloid cells in vitro and the activity of increasing the number of platelets in the peripheral vessel in vivo, and purifying the factor. The obtained colony stimulating factor is a protein having a molecular weight of 24,000 and an isoelectric point of 4.5 to 5.5, and is useful for treating a certain kind of platelet reducing diseases. This factor can be mixed with conventional various excipients to form medicinal compositions in various preparation forms.
Bibliography:Application Number: DE19896028138T