VERSTAERKTE PRODUKTION VON LIPIDEN ENTHALTEND MEHRFACHUNGESAETTIGTE FETTSAEUREN DURCH HOCHDICHTE KULTUREN VON EUKARIOTISCHEN MIKROBEN IN GAERVORRICHTUNGEN

• Main Authors: BAILEY, RICHARD B, DIMASI, DON, HANSEN, JON M, KANEKO, TATSUO, MIRRASOUL, PETER J, BARCLAY, WILLIAM R, WEEDER, GEORGE T, RUECKER, CRAIG M
• Format: Patent
• Language: German
• Published: 30.10.2008
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKINGOILS; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT,VEGETABLES, EDIBLE SEEDS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THE PRESERVED, RIPENED, OR CANNED PRODUCTS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

A process for growing eukaryotic microorganisms comprising adding to a fermentation medium comprising said microorganisms a carbon source and a limiting nutrient source at a rate sufficient to increase the biomass density of said fermentation medium to at least about 100 g/L, wherein said microorganisms are selected from the group consisting of algae, fungi (including yeast), protists, bacteria and mixtures thereof, and wherein said microorganisms are capable of producing at least about 20% of their biomass as lipids comprising some polyenoic fatty acids.