METHODE ZUR HERSTELLUNG VON POLYHYDROXYALKANOATEN IN REKOMBINANTEN ORGANISMEN

The present biotechnological approach for the production of polyhydroxyalkanotes (PHAs) uses microbial systems. The high production costs makes them substantially more expensive than synthetic plastics. Engineering a novel pathway in eucaryotic cell systems is a beneficial alternative to the product...

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Bibliographic Details
Main Authors AQUIN, STEPHANIE, VEZINA, LOUIS-P
Format Patent
LanguageGerman
Published 01.06.2006
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TRAPPING
VINEGAR
WINE
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Summary:The present biotechnological approach for the production of polyhydroxyalkanotes (PHAs) uses microbial systems. The high production costs makes them substantially more expensive than synthetic plastics. Engineering a novel pathway in eucaryotic cell systems is a beneficial alternative to the production of PHAs in bacteria. This pathway will initially produce free (C8) fatty acids from the fatty acid synthetic pathway through the action of thioesterase, that will then add a CoA moiety to the free fatty acid through the action of an acyl-CoA synthetase, that will produce 3-ketoacyl-CoAs from the acyl-CoA through the action of a thiolase, that will produce R-(-)-OH-acyl-CoAs from the 3-keto acid CoAs through the action of a dehydrogenase isoform from yeasts. These R-(-)-3-OH-acyl-CoAs will finally be used as substrate for the PHA synthase reaction.
Bibliography:Application Number: DE20006021537T