PICHIA METHANOLICA GLYCERALDEHYD-3-PHOSPHAT-DEHYDROGENASE-1-PROMOTER UND -TERMINATOR

Transcription promoter and terminator sequences from the Pichia methanolica glyceraldehyde-3-phosphate dehydrogenase 1 gene (GAP1 gene) are disclosed. The sequences are useful within DNA constructs for the production of proteins of interest in cultured P. methanolica cells. Within the expression vec...

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Bibliographic Details
Main Authors RAYMOND, K, MILLER, G, SLOAN, S, VANAJA, ERICA
Format Patent
LanguageGerman
Published 22.09.2005
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Transcription promoter and terminator sequences from the Pichia methanolica glyceraldehyde-3-phosphate dehydrogenase 1 gene (GAP1 gene) are disclosed. The sequences are useful within DNA constructs for the production of proteins of interest in cultured P. methanolica cells. Within the expression vectors, a GAP1 promoter and/or a GAP1 terminator is operably linked to a DNA segment encoding the protein of interest.
Bibliography:Application Number: DE20006013829T