Increased prodn. of poly:hydroxy:alkanoate in prokaryotic cells

In the prodn. of polyhydroxyalkanoates (I) in a prokaryotic host, contg. the genes required for (I) synthesis, by culturing on a medium under appropriate conditions, the new features are that the host contains at least one copy of a (I)-synthase gene (II) under increased expression control and the m...

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Bibliographic Details
Main Authors LUBITZ, WERNER., WIEN, AT, RESCH, STEFANIE., WIEN, AT
Format Patent
LanguageEnglish
German
Published 23.11.1995
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS BASED THEREON
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC MACROMOLECULAR COMPOUNDS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
THEIR PREPARATION OR CHEMICAL WORKING-UP
VINEGAR
WINE
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Summary:In the prodn. of polyhydroxyalkanoates (I) in a prokaryotic host, contg. the genes required for (I) synthesis, by culturing on a medium under appropriate conditions, the new features are that the host contains at least one copy of a (I)-synthase gene (II) under increased expression control and the medium is practically free of glucose. Also new are: (1) recombinant DNA (III) contg. (II) under increased expression control; (2) recombinant vectors contg. at least one copy of (III); and (3) prokaryotic cells contg. (III) or these vectors.
Bibliography:Application Number: DE19944417169