NACHWEIS VON INFLUENZA-A-VIRUS DURCH POLYMERASE-KETTENREAKTION (PCR) NACH REVERSER TRANSKRIPTION EINES BEREICHS DES VIRALEN HAEMAGGLUTININ-GENS

The sensitivity of detection of influenza A virus has been considerably increased by carrying out a polymerase chain reaction (PCR) using oligonucleotide sequences (amplimers) from the reasonably conserved region of the viral haemagglutinin gene after previous reverse transcription (RT). The reverse...

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Bibliographic Details
Main Authors CERUTTI, PETER A., , PULLY, LAUSANNE, CH, BRESSOUD, ALBERIC, LAUSANNE, CH
Format Patent
LanguageGerman
Published 07.03.1991
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:The sensitivity of detection of influenza A virus has been considerably increased by carrying out a polymerase chain reaction (PCR) using oligonucleotide sequences (amplimers) from the reasonably conserved region of the viral haemagglutinin gene after previous reverse transcription (RT). The reverse transcription and the PCR are in this case insensitive to single mutations by base exchange unless the 3' end of the amplimers is affected.
Bibliography:Application Number: DE19893929144