GENTECHNOLOGISCHES VERFAHREN ZUR HERSTELLUNG VON ANGIOGENINEN

Fusion proteins with beta -galactosidase or a beta -galactosidase fragment with more than 250 amino acids but significantly fewer than the complete sequence of beta -galactosidase, where interfering methionines and/or arginines and/or cysteines in the beta -galactosidase portion have been replaced b...

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Bibliographic Details
Main Authors HEIN,FRIEDRICH,DR, CRAUSE,PETER,DR, UHLMANN,EUGEN,DR, WILLI,JANSEN,HANS
Format Patent
LanguageGerman
Published 01.12.1988
Edition4
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Fusion proteins with beta -galactosidase or a beta -galactosidase fragment with more than 250 amino acids but significantly fewer than the complete sequence of beta -galactosidase, where interfering methionines and/or arginines and/or cysteines in the beta -galactosidase portion have been replaced by other amino acids, and with an angiogenin are insoluble and easy to isolate from bacterial cells. Angiogenin and its derivatives can therefore be obtained by coupling the structural gene for the required polypeptide in the correct reading frame, if required via an adaptor, to a gene for such a beta -galactosidase derivative via a regulatory region, expressing the insoluble fusion protein in a bacterium, isolating the protein after a cell disruption, and liberating the required angiogenin by chemical or enzymatic cleavage.
Bibliography:Application Number: DE19873716722