KONTRASTVERSTAERKUNG FUER DEN NACHWEIS VON IMMUNOLOGISCHEN FILMEN

• Main Author: GOLIBERSUCH, DAVID CLARENCE, SCHENECTADY, N.Y. (V.ST.A.)
• Format: Patent
• Language: German
• Published: 27.02.1975
• Subjects: CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; PHYSICS; TESTING

A method for increasing the sensitivity of immunologic diagnostic slides by increasing the contrast observable between immunologically reacted and unreacted portions thereof. The method is based on the fact that an arbitrary antibody functions as an antigen when introduced into the system of a vertebrate to whom it is a foreign protein. Specifically reactive antibodies to the antibodies so introduced are therefore produced and may be collected. Accordingly, a plurality of specific immunological reactions may be performed in chain fashion. This technique is used in accordance with one embodiment to build a plurality of successive antigen-antibody films on a substrate to provide for contrast between multilayer protein films and a monomolecular layer which is in excess of the contrast obtainable between monomolecular layers and bimolecular layers to thereby provide diagnostic apparatus of increased sensitivity.