DE1917057

• Main Authors: POWELL, LAWSON WILLIAM, WORTHING, SUSSEX, GB, CARRINGTON, THOMAS RONALD, GORING-BY-SEA, SUSSEX, GB, SAVIDGE, THOMAS ADRIAN, STEYNING, SUSSEX, GB
• Format: Patent
• Language: English
• Published: 08.09.1983
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HETEROCYCLIC COMPOUNDS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

(A) A water insoluble enzyme prepn. (I) (B) Improved method for 6-aminopenicillanic acid. Deacylation of penicillin. A suitable acylase, obtd. e.g. from E. coli or fungal sources, is reacted with a suitable reactive carboxymethylcellulose, Zeocarb 226 acid chloride, or hydrazine cross-linked ethylene-maleic anhydride resins, tog. with the appropriate amide-forming reagent, e.g. DCCD. The coupled enzyme is esp. useful of for deacylation of benzyl and phenoxymethylpenicillins to 6-aminopenicillanic acid of high purity.