Culturing Rhodococcus rhodochrous for preparation of nitrile hydratase, useful for converting nitriles to amides, particularly preparation of acrylamide, by growing in phosphate-buffered medium of pH 5.5 to 9

• Main Authors: WORONIN, S.P, LORENZ, JOSEF, YANENKO, A.S, SINGIRZEV, I.N, KOSULIN, S.W, DEBABOV, V.G
• Format: Patent
• Language: English; German
• Published: 04.11.2004
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Method for culturing the nitrile hydratase (I)-producing strain Rhodococcus rhodochrousM33 (DSM 14230) using a medium based on 12-60 mM phosphate buffer that (a) satisfies the cells' requirement for phosphorous and (b) maintains pH 5.5-9 during culture. An independent claim is also included for a medium for the process comprising, per l, 7.9 g sodium dihydrogenphosphate dodecahydrate; 1.8 g potassium dihydrogenphosphate; 0.02-0.04 g cobalt(II) chloride hexahydrate; 1 g magnesium sulfate heptahydrate; 0-10 g maize extract; 20-90 g glucose and 4-20 g urea. Die Erfindung betrifft ein Verfahren zur Kultivierung des Nitrilhydratase-produzierenden Stammes Rhodococcus rhodochrous M33, wobei ein Kultivierungsmedium verwendet wird, das auf einem 12-60 mM Phosphat-Puffer basiert und den Bedarf der Zellen an Phosphor deckt, den pH-Wert während der Kultivierung im Bereich von 5,5 bis 9,0 aufrechterhält, sowie das im Verfahren verwendete Kultivierungsmedium.