Verfahren sowie Mikroorganismen zur mikrobiellen Herstellung von Pyruvat aus Kohlenhydraten sowie Alkoholen

• Main Authors: TAKORS, RALF, BOTT, MICHAEL, DR, GERHARZ, TANJA, ZELIC, BRUNO
• Format: Patent
• Language: German
• Published: 23.05.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Verfahren zur mikrobiellen Herstellung von Pyruvat aus Kohlenhydraten sowie Alkoholen unter Verwendung von Mikroorganismen, in denen die Pyruvat-Dehydrogenase, die Phosphoenolpyruvat-Synthetase sowie die Pyruvat-Oxidase inaktiviert sind und/oder eine verringerte Aktivität aufweisen, dadurch gekennzeichnet, dass die D-Lactat-Dehydrogenase ausgeschaltet wird und diese Mikroorganismen eingesetzt werden. The invention relates to a method and micro-organisms for the microbial production of pyruvate from carbohydrates and alcohols. Known chemical methods for producing pyruvate are technically complex and cost-intensive. Known biological methods and micro-organisms have a drawback in that the substrates are not 100 % converted into pyruvate thereby resulting in a deterioration of the product yield. In addition, a complex control of the necessary vitamin concentration is required for the microbial pyruvate production from glucose. The inventive method and micro-organisms enable the microbial production of pyruvate with a nearly 100 % conversion of the substrate. An increased pyruvate production could be achieved by using micro-organisms that have been genetically modified, i.e. deletion, insertion or low expression of the ldhA gene sequence, which codes for the lactate dehydrogenase.