In vitro automatic screening procedure for the determination of the developmental neurotoxicity of noxae on normal human neural brain cells, comprises culturing neurospheres under removal of growth factors and under addition of noxae

• Main Authors: ABEL, JOSEF, KURTMANN, JEAN, FRITSCHE, ELLEN, MOORS, MICHAELA
• Format: Patent
• Language: English; German
• Published: 09.08.2007
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The In vitro automatic screening procedure for the determination of the developmental neurotoxicity (DNT) of noxae on normal human neural brain cells, comprises culturing neurospheres (1) under removal of growth factors and under addition of the concentrated noxae and immobilizing on coated slides, and microscopically determining the distance and/or the number of cells, which grow from the neurospheres in a time and which migrate radially from the spheres edge, from the sphere core and comparing with the distance of the migrating cells and/or their count. The In vitro automatic screening procedure for the determination of the developmental neurotoxicity (DNT) of noxae on normal human neural brain cells, comprises culturing neurospheres (1) under removal of growth factors and under addition of the concentrated noxae and immobilizing on coated slides, and microscopically determining the distance and/or the number of cells, which grow from the neurospheres in a time and which migrate radially from the spheres edge, from the sphere core and comparing with the distance of the migrating cells and/or their count, achieved under identical culture conditions in noxae-free cultures. The brain cells form functional neural networks during their natural development in the brain. The distance of the fully grown and toxically unaffected cells radially from the cell boundary is 0.3-0.4 mm, after a culturing time of 24 hours. Die Erfindung betrifft ein In-vitro-Verfahren zur Bestimmung der Entwicklungsneurotoxizität von Noxen auf normale humane neurale Gehirnzellen durch Messung der Migrationsstrecke und Anzahl migrierter Zellen vom auf Objektträgern fixierten Neurosphären.