New nucleic acid encoding plant phosphomevalonate kinase, useful for identifying modulators, potentially useful as herbicides and growth regulators

• Main Authors: MEISSNER, RUTH, LECHELT-KUNZE, CHRISTA
• Format: Patent
• Language: English; German
• Published: 23.05.2002
• Edition: 7
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUSPLANTS; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CATCHING, TRAPPING OR SCARING OF ANIMALS; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PHYSICS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; TRAPPING; VINEGAR; WINE

A nucleic acid (I) that encodes a plant phosphomevalonate kinase (PMVK), excluding the known fully defined partial sequences (S3-5) of 611, 728 and 571 base pairs as given in the specification, is new. Independent claims are also included for the following: (a) DNA construct (DC) comprising (I) and a heterologous promoter; (b) vector containing (I) or DC; (c) host cell containing (I), DC or the vector of (b); (d) polypeptide (II) with PMVK activity encoded by (I); (e) antibody (Ab) that binds specifically to (II); (f) preparing (I) or (II); (g) identifying compounds (A) that bind to and/or modulate activity of (II); (h) identifying compounds (B) that alter expression of (II); and (i) (A), (B) and herbicides identified by methods (g) and (h). Die Erfindung betrifft Nukleinsäuren, die für pflanzliche Polypeptide mit der biologischen Aktivität von Phosphomevalonat Kinasen kodieren, die davon die kodierten Polypeptide sowie deren Verwendung als Targets für Herbizide und deren Verwendung zum Identifizieren von neuen, herbizidwirksamen Verbindungen sowie Verfahren zum Auffinden von Modulatoren dieser Polypeptide.