VERFAHREN ZUR REINIGUNG VON NUCLEOTIDSEQUENZEN

A method for obtaining a strain of microorganisms modified to contain a fragment of a specific deoxyribonucleotide sequence, which method comprises: (I) purifying a fragment of a deoxyribonucleotide sequence by the following steps: (a) providing a population of cdna transcripts of the polyribonucleo...

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Bibliographic Details
Main Authors SHINE,JOHN,AU, GOODMANN,HOWARD M.,US, SEEBURG,PETER H.,DE
Format Patent
LanguageGerman
Published 06.01.1982
Edition3
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DERIVATIVES THEREOF
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
SUGARS
VINEGAR
WINE
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Summary:A method for obtaining a strain of microorganisms modified to contain a fragment of a specific deoxyribonucleotide sequence, which method comprises: (I) purifying a fragment of a deoxyribonucleotide sequence by the following steps: (a) providing a population of cdna transcripts of the polyribonucleotides. (b) submit the cdna transcripts to the action of a restriction endonuclease preparation capable of catalyzing the hydrolysis of the cdna transcripts at each of the restriction sites. (c) fractionating the fragments produced by the action of the restriction endonuclease according to its length. (d) pretreating the dna to remove any 5''-phosphate terminal group. (e) incubating the dna with a restriction endonucelase capable of acting on the desired specific nucleotide sequence to produce two linear sub-fragments thereof. (f) fractionating the sub-fragments according to their length. (g) isolate the two sub-fragments, (h) assemble the sub-fragments covalently, in a reaction catalyzed by dna ligase, (I) fractionate the assembled dna molecules according to their length, (II) recombine the resulting product of the above sequence with a dna transfer vector and transfer it to a microorganism. (Machine-translation by Google Translate, not legally binding)
Bibliography:Application Number: DD19780224374