Post-column derivatization with solid derivatization agent in HPLC or CLC

• Main Authors: TESA OVÁ EVA, KUBÍ EK VOJT CH, COUFAL PAVEL, FRANC MARTIN, VOJTA JI Í, KUBÍ KOVÁ ANNA, BOSÁKOVÁ ZUZANA
• Format: Patent
• Language: Czech; English
• Published: 13.05.2015
• Subjects: ACYCLIC OR CARBOCYCLIC COMPOUNDS; CHEMISTRY; COLORIMETRY; COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSESC01D OR C01F; INORGANIC CHEMISTRY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT,POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED,VISIBLE OR ULTRA-VIOLET LIGHT; MEASURING; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; RADIATION PYROMETRY; TESTING

Řešení se týká detekce dusíkatých látek (aminokyseliny, peptidy, aminy a jejich deriváty) pomocí "on-line" postkolonové derivatizace tuhým derivatizačním činidlem (měď anebo nerozpustné sloučeniny mědi) pro spektrofotometrickou detekci v ultrafialové a viditelné (UV-VIS) oblasti spektra. Derivatizace tuhým činidlem a následná spektrofotometrická detekce je využitelná ve vysoce účinných separačních metodách, jako je vysokoúčinná kapalinová chromatografie (HPLC) a kapilární kapalinová chromatografie (CLC). The present invention relates to a detection of nitrogenous substances (amino acids, peptides, amines and derivatives thereof) by means of "on-line" post-column derivatization with a solid derivatization agent (copper or insoluble copper compounds) for spectrophotometric detection in ultraviolet and visible (UV-VIS) spectra. The derivatization with a solid derivatization agent followed by spectrophotometric detection is usable in high-effective separation methods such as High Performance Liquid Chromatography (HPLC) and Capillary Liquid Chromatography (CLC).