Modified Cry3A protein for killing bark beetles and darkling beetles and method of its production

• Main Authors: SEHNAL FRANTISEK, MALA JANA, PAVINGEROVA DANIELA, VLASAK JOSEF, BRIZA JINDRICH
• Format: Patent
• Language: Czech; English
• Published: 20.06.2012
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

Rešení popisuje rekombinantní protein pripravený ze syntetického genu odvozeného z Bacillus thuringiensis var. tenebrionis Cry3A, který byl rekonstruován pro vyšší toxicitu úpravami kritické smycky 1 proteinové domény II. Dále je popsána jeho produkce a použití jako úcinného insekticidu proti kurovci smrkovému (Ips typographus) a potemníku hnedému (Tribolium castaneum).