Shortened derivatives of human insulin
In the present invention, there are disclosed human insulin shortened derivatives of the general formula I X - Gly - Y (I), wherein X represents insulin desoctapeptide, Y denotes Phe-Phe-PheNHi2 or Phe-Phe-N(CHi3)PheNHi2 or Phe-N(CHi3)-Phe-PheNHi2. Disclosed is also a process for preparing such deri...
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Main Authors | , , , , , , , , , , , , |
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Format | Patent |
Language | Czech English |
Published |
12.09.2001
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Edition | 7 |
Subjects | |
Online Access | Get full text |
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Summary: | In the present invention, there are disclosed human insulin shortened derivatives of the general formula I X - Gly - Y (I), wherein X represents insulin desoctapeptide, Y denotes Phe-Phe-PheNHi2 or Phe-Phe-N(CHi3)PheNHi2 or Phe-N(CHi3)-Phe-PheNHi2. Disclosed is also a process for preparing such derivatives, wherein the insulin desoctapeptide is condensed with tetrapeptides of the general formula Gly-Phe-Phe-Phe-NHi2 or Gly-Phe-Phe-N(CHi3)PheNHi2 or Gly-Phe-N(CHi3)-Phe-PheNHi2 in the presence of trypsin tosylphenyl alaminochloroketone in 50 - 65 percent dimethylformamide at a temperature ranging from 4 to 25 degC for a period of 3 to 24 hours, whereupon the obtained products are isolated, especially using high-performance liquid chromatography to reverse phase. |
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Bibliography: | Application Number: CZ20010020004 |