Shortened derivatives of human insulin

In the present invention, there are disclosed human insulin shortened derivatives of the general formula I X - Gly - Y (I), wherein X represents insulin desoctapeptide, Y denotes Phe-Phe-PheNHi2 or Phe-Phe-N(CHi3)PheNHi2 or Phe-N(CHi3)-Phe-PheNHi2. Disclosed is also a process for preparing such deri...

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Main Authors KLASOVA LENKA MGR, SKARDA JOSEF MVDR.SC, KASICKA VACLAV RNCSC, VELEK JIRI CSC, WOLLMER AXEL DR, BRANDENBURG DIETRICH DR, ZORAD STEFAN CSC, BARTHOVA JANA DOC. RNCSC, VELKOVA VLASTA RNDR, JEZEK JAN RNCSC, HUML KAREL RNDRSC, UBIK KAREL CSC, BARTH TOMISLAV RNDRSC
Format Patent
LanguageCzech
English
Published 12.09.2001
Edition7
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Summary:In the present invention, there are disclosed human insulin shortened derivatives of the general formula I X - Gly - Y (I), wherein X represents insulin desoctapeptide, Y denotes Phe-Phe-PheNHi2 or Phe-Phe-N(CHi3)PheNHi2 or Phe-N(CHi3)-Phe-PheNHi2. Disclosed is also a process for preparing such derivatives, wherein the insulin desoctapeptide is condensed with tetrapeptides of the general formula Gly-Phe-Phe-Phe-NHi2 or Gly-Phe-Phe-N(CHi3)PheNHi2 or Gly-Phe-N(CHi3)-Phe-PheNHi2 in the presence of trypsin tosylphenyl alaminochloroketone in 50 - 65 percent dimethylformamide at a temperature ranging from 4 to 25 degC for a period of 3 to 24 hours, whereupon the obtained products are isolated, especially using high-performance liquid chromatography to reverse phase.
Bibliography:Application Number: CZ20010020004