METHOD OF NEW SEED PRODUCTION FOR B 12 COENZYME PRODUCTION

A fermentation process is disclosed for the preparation of an inoculum, suitable for batchwise, semicontinuous, or continuous production of coenzyme B12 under septic conditions. The fermentation process begins with a broth consisting of methanol, cornsteep liquor hydrolysate or heat-treated corn slo...

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Bibliographic Details
Main Authors UDVARDY NAGY EVA DR.,HU, SZEMLER LASZLO ,HU
Format Patent
LanguageEnglish
Published 11.04.1991
Edition5
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A fermentation process is disclosed for the preparation of an inoculum, suitable for batchwise, semicontinuous, or continuous production of coenzyme B12 under septic conditions. The fermentation process begins with a broth consisting of methanol, cornsteep liquor hydrolysate or heat-treated corn slop, ammonium bicarbonate, magnesium chloride, cobalt chloride, 5,6-dimethyl-benzimidazole and sodium bisulfite used for anaerobic, mesophilic, septic fermentation production of coenzyme B12 with anaerobic, digested sewage sludge. Fermentation is carried out over several steps to provide an inoculum containing an anaerobic, mesophilic, methane-producing, new mixed micropopulation, containing bacteria of each of the types deposited at the Hungarian National Collection of Medical Bacteria, National Institute of Hygiene, under numbers 00076, 00079, and 00272.
Bibliography:Application Number: CS19840006931