STRAIN OF MICROORGANISM BREVIBACTERIUM FLAVUM CCM 4126

The purpose of the solution consists in production of essential aminoacid of lysine, used mainly as an additive into fodder mixtures for feeding economically useful animals, mainly piggery. Lysine is synthesized and accumulated in medium by mutant of bacterial strand Brevibacterium flavum, isolated...

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Main Authors HANO ALEXANDER,CS, PLACHY JIRI RNCSC.,CS, PILAT PETR CSC.,CS, BUCKO MICHAL DRSC.,CS, ULBERT STANISLAV ,CS, CULIK KAREL RNDR.,CS, BOROSOVA GABRIELA ,CS
Format Patent
LanguageEnglish
Published 12.11.1991
Edition5
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The purpose of the solution consists in production of essential aminoacid of lysine, used mainly as an additive into fodder mixtures for feeding economically useful animals, mainly piggery. Lysine is synthesized and accumulated in medium by mutant of bacterial strand Brevibacterium flavum, isolated after induction by mutagen using selective media. The mutant, resistant against 5-(2-aminoethyl)-L-cysteine, dependant on homoserine, leucine and sensitive towards 1ug/ml of sodium 3-fluorpyruvate, cultivated in medium with saccharose as the source of carbon, hydrolysate of peanut flour and corn-steep liquor as the source of organic nitrogen and growth factors, with ammonium sulphate as the source of inorganic nitrogen and with mineral salts, cumulated during conversion 42 percent in aerobic conditions at the temperature of 28 degrees C and neutral pH of the environment 55g/L of L-lysine. The lysine can be produced in this manner using standard technological procedures.
Bibliography:Application Number: CS19890003352