Negative pressure balance centrifugal bottle capable of preventing cell stacking sheet preparation

• Main Author: ZHU SILONG
• Format: Patent
• Language: Chinese; English
• Published: 23.08.2022
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

The utility model provides a negative pressure balance centrifugal bottle for preventing cell stacking sheet preparation, which comprises a centrifugal bottle body, a centrifugal bottle cap and a main conical tube, the small end of the main conical tube is connected with the large end of a buffer conical tube, the small end of the buffer conical tube is connected with a thin tube, the inner diameter of the thin tube is 1-3mm, and the inner diameter of the thin tube is 1-3mm. The included angle between the generatrix of the buffering conical pipe and the shaft is 30-50 degrees. According to the negative pressure balance centrifugal bottle for preventing cell stacking slide preparation, the smooth buffering conical tube and the smooth transition conical tube are arranged before cells enter the thin tube, only a small number of cells can directly enter the thin tube to reach the glass slide at any centrifugal speed and under the action of centrifugal force, and the cell stacking is prevented. Part of cells enter