Micro-droplet sampling device applied to digital PCR imaging method detection and system thereof

• Main Authors: DU XIAOCHUN, BAI YU, JING GAOSHAN, SHENG TIANCHENG, GUO YONG, SU SHISHENG, WANG BO, PENG YANKE
• Format: Patent
• Language: Chinese; English
• Published: 30.06.2020
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

The utility model provides a micro-droplet sampling device and a micro-droplet sampling system applied to digital PCR imaging method detection, the micro-droplet sampling device comprises a micro-droplet floating module and a micro-droplet storage module, and the micro-droplet floating module and the micro-droplet storage module are hermetically connected; a micro-droplet tiling chamber is arranged on the surface of the micro-droplet floating module, and micro-droplets in the micro-droplet storage module enter the micro-droplet tiling chamber in batches to realize single-layer tiling and imaging detection of the micro-droplets. According to the utility model, batch sampling of a micro-droplet sample is realized, the sensitivity and reliability of digital PCR detection are improved, and theflux of micro-droplet detection is increased. 本实用新型提供一种数字PCR成像法检测中应用的微液滴取样装置以及其系统，微液滴取样装置包括微液滴上浮模块和微液滴存储模块，两者之间密封连接；所述微液滴上浮模块表面设置有微液滴平铺室，所述微液滴存储模块中的微液滴分批进入到所述微液滴平铺室中实现微液滴单层平铺和成像检测。本实用新型实现了对一个微液滴样本的分批次取样，提高了数字PCR检测的灵敏度与可靠度和提高