Microfluidic chip for multi-fluorescence quantitative PCR (Polymerase Chain Reaction) detection of canine viruses

• Main Authors: QIN TONG, CUI SHANGJIN
• Format: Patent
• Language: Chinese; English
• Published: 22.11.2019
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The utility model relates to a canine virus multi-fluorescence quantitative PCR (Polymerase Chain Reaction) detection micro-fluidic chip. The canine virus multi-fluorescence quantitative PCR detectionmicro-fluidic chip consists of a substrate, an upper cover plate and a lower cover plate, wherein a sample injection hole A, a micro-channel, a DNA separation chamber, a triangular valve, a waste liquid chamber, a sample injection hole B and a PCR reaction chamber are arranged on the substrate; positions corresponding to the sample injection hole A, the sample injection hole B and the triangularvalve are reserved on the upper cover plate, and a convex open chamber is arranged at a position corresponding to the waste liquid chamber and is bonded on the upper surface of the base plate; the lower cover plate is bonded to the lower surface of the substrate to seal the lower surface of the substrate. According to the utility model, DNA separation and multiple fluorescent quantitative PCR detection can be realized on o