Recombinant alpha-fetoprotein, method and means for preparation thereof, compositions on the base thereof and use thereof

• Main Authors: DUDICH DMITRY I, DUDICH ELENA I, DUDICH IGOR V, ZATSEPIN SERGEI SERGEEVICH, SHINGAROVA LYUDMILA NIKOLAEVNA, BENEVOLENSKY SERGEI VLADIMIROV, TATULOV EDUARD BORISOVICH, MARCHENKO ALEXEI NIKOLAEVICH, KOZLOV DMITRY GEORGIEVICH, SEMENKOVA LIDIYA N
• Format: Patent
• Language: Chinese; English
• Published: 20.07.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to the microbiological and medical industry, genetic engineering, biotechnology. A Saccharomyces cerevisiae yeast strain was obtained on the basis of constructing a recombinant plasmid DNA comprising a structural gene of a human alpha-fetoprotein (AFP) under the control of a regulatory promoter, providing the synthesis and production of AFP in a secreted soluble form, this AFP having activity identical or similar to the activity of a human AFP. The obtained recombinant AFP may be used as an active substance for the preparation of therapeutic agents for use in oncology, immunotherapy, cosmetology and also for the diagnosis of cancer and embryonic pathologies.