Measles virus fluorescent augmentation detection kit and detection method

The present invention provides a kind of measles virus fluorescence amplification detection kit and method for detecting measles virus by using said kit. Said kit includes measles virus hemagglutinin gene standard product, fluorescence amplification detection reagent and DNA polymerase and reverse t...

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Bibliographic Details
Main Author YIYU,YAN LU
Format Patent
LanguageEnglish
Published 31.01.2007
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:The present invention provides a kind of measles virus fluorescence amplification detection kit and method for detecting measles virus by using said kit. Said kit includes measles virus hemagglutinin gene standard product, fluorescence amplification detection reagent and DNA polymerase and reverse transcriplase. The fluorescence amplification detection reagent mainly includes one-step method RT-PCR buffer fluid, specific amplification primer and specific probe and deoxynucleoside triphosphate mixture. Said method can directly detect pathogen nucleic acid, and can detect the specific gene containing measles virus.
Bibliography:Application Number: CN2006150678