H5N1 type poultry grippal virus fluorescent augmentation detection kit and detection method

The present invention provides a kind of H5N1-type avian influenza virus fluorescence amplification detection kit and detection method for detecting avian influenza virus byusing said kit. Said kit includes the following several portions: H5N1-type avian influenza virus hemagglutinin gene standard p...

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Bibliographic Details
Main Author YIYU,YAN LU
Format Patent
LanguageEnglish
Published 31.01.2007
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:The present invention provides a kind of H5N1-type avian influenza virus fluorescence amplification detection kit and detection method for detecting avian influenza virus byusing said kit. Said kit includes the following several portions: H5N1-type avian influenza virus hemagglutinin gene standard product, fluorescence amplification detection reagent and DNA polymerase and reverse transcriptase. The fluorescence amplification detection reagent mainly includes one-step method RT-PCR buffer fluid, specific amplification primer and specific probe and deoxynucleoside triphosphate mixture. Said method can directly detect pathogen nucleic acid, and can detect specific gene containing H5N1-type avian influenza virus.
Bibliography:Application Number: CN2006150675