Surfactant-tolerant cellulase and method of converting the same

Disclosed is a method of inhibiting a lowering in endoglucanase activity in the presence of a surfactant characterized by comprising modifying a protein having the endoglucanase activity and carrying no pyroglutamic acid at the N-end into another protein carrying pyroglutamic acid at the N-end. In a...

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Bibliographic Details
Main Authors TSUYUKI YUMIKO, WATANABE MANABU, YANAI KOJI
Format Patent
LanguageChinese
English
Published 14.11.2012
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Disclosed is a method of inhibiting a lowering in endoglucanase activity in the presence of a surfactant characterized by comprising modifying a protein having the endoglucanase activity and carrying no pyroglutamic acid at the N-end into another protein carrying pyroglutamic acid at the N-end. In addition, disclosed are a protein having endoglucanase activity and having been modified into pyroglutamic acid at the N-end by amino acid modification; a polynucleotide encoding the above protein; an expression vector containing the above polynucleotide; a host cell transformed by the above expression vector; and a process for producing the above protein by culturing the above host cell.
Bibliography:Application Number: CN200480036453