Process for producing a-glycosylated dipeptide and method of assaying a-glycosylated dipeptide

• Main Author: HIROKAWA KOZO,KUROSAWA KEIKO,KAJIYAMA NAOKI
• Format: Patent
• Language: English
• Published: 25.10.2006
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

A process for producing an alpha-glycosylated dipeptide characterized in that a protease acts on a peptide having its N-terminus glycosylated or protein having its N-terminus glycosylated; and a method of assaying an alpha-glycosylated dipeptide characterized in that fructosyl-peptide oxidase acts on the alpha-glycosylated dipeptide obtained by the process so as to generate hydrogen peroxide and the thus generated hydrogen peroxide is measured. The provided process for producing an alpha-glycosylated dipeptide realizes simple, speedy and efficient production of an alpha-glycosylated dipeptide from a glycosylated protein or glycosylated peptide. The provided method of assaying an alpha-glycosylated dipeptide realizes short-time accurate quantitative determination of alpha-glycosylated dipeptide.