Cultivation method for highly effective plant tissue differentiation and regeneration

The present invention relates to tissue culture method with high efficiency induction of plant tissue regeneration, and features that during plant callus induction and differentiation, betaine aldehyde is added into the culture medium to raise the differentiation and regeneration rate of callus bud,...

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Bibliographic Details
Main Author ZANMIN,LI HU
Format Patent
LanguageEnglish
Published 27.09.2006
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Summary:The present invention relates to tissue culture method with high efficiency induction of plant tissue regeneration, and features that during plant callus induction and differentiation, betaine aldehyde is added into the culture medium to raise the differentiation and regeneration rate of callus bud, shorten the regeneration bud obtaining period and raise the growth speed of the regenerated bud effectively. The said method can raise the regeneration rate of callus by over 1 time, move up the germination time by 5 days and shorten the culture period from the inoculation of explant to transformation to the rooting culture medium by 10-15 days. The present invention also relates to the callus or contagious bud obtained by means of the said method as well as plant obtained through differentiation of the callus or contagious bud.
Bibliography:Application Number: CN2005159187