Domestication method for lotus root group cultivating de-poisoning breeding

The invention provides a domestication method for lotus root group cultivating de-poisoning breeding which comprises, using tips of lotus rhizome stem as explant to inoculate and culture non-bacterial vaccine, switching to culture medium for relay generation propagation, transfereing the culture spr...

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Bibliographic Details
Main Authors YOUWEI ZHAO, BEISHENG CAO, LIANGJUN LI
Format Patent
LanguageEnglish
Published 29.06.2005
Edition7
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED
FISHING
FORESTRY
HORTICULTURE
HUMAN NECESSITIES
HUNTING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
TRAPPING
WATERING
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Summary:The invention provides a domestication method for lotus root group cultivating de-poisoning breeding which comprises, using tips of lotus rhizome stem as explant to inoculate and culture non-bacterial vaccine, switching to culture medium for relay generation propagation, transfereing the culture sprouts into 1-3 times of MS or B-5 minimum medium, charging into culture medium of 4-15% of cane sugar and 0-1000 mg/L of penicillin, disposing 7-15 days, steeping and flushing the lotus rhizome cuture sprouts with tap water, processing 2-48 hours with 50-5000mg/L of choline chloride, dipping 50-1000 mg/lNAA and transplanting into shallow water field directly. The invention can realize a survival rate of over 90%.
Bibliography:Application Number: CN2004165333