Glass freezing preserving method for fish embryo

• Main Authors: YONGSHENG TIAN, SONGLIN CHEN
• Format: Patent
• Language: Chinese; English
• Published: 08.12.2004
• Edition: 7
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; FORESTRY; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; HUMAN NECESSITIES; HUNTING; NEW BREEDS OF ANIMALS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; TRAPPING

A vitrifying freeze storage method for the embryos of fish, especially the bastard halibut, is disclosed, which features that the formula of vitrifying liquid suitable for the embryo of seawater fish is screened out, the embryo development stage suitable for vitrifying it is determined, a particular eluting solution is disclosed, and the step balance and the fast cooling and thawing techniques are used. Its advantages are high safety, easy operation and low cost. 一种鱼类胚胎玻璃化冷冻保存方法，通过比较不同抗冻剂的毒性效应和玻璃化性能，筛选出适宜海水鱼类胚胎的玻璃化液配方；确定适宜玻璃化处理的胚胎发育时期，研制出特定的洗脱溶液，采用分步平衡、快速降温和解冻的技术路线，建立了鱼类胚胎玻璃化冷冻保存技术。对牙鲆不同发育时期胚胎进行了8次玻璃化冷冻实验中共获得冷冻复活胚20粒，最高复活率达32％。其中5次实验中获得冷冻复活胚胎发育孵化出鱼苗的结果。在20枚冷冻复活胚胎中，有14枚冻胚孵化出鱼苗，孵化率高达70％。冻胚孵出的鱼苗，胚体透明，形态正常，无畸形。本发明技术无需昂贵的仪器设备，操作简单，成本低廉，易于推广，可用于牙鲆等海水鱼类胚胎超低温(-196℃)冷冻保存。