Guluronicacid oligosaccharides production method utilizing Fenton-type reaction

• Main Author: HUASHI,YANG GUAN
• Format: Patent
• Language: Chinese; English
• Published: 10.11.2004
• Edition: 7
• Subjects: CHEMISTRY; DERIVATIVES THEREOF; METALLURGY; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; SUGARS

A process for preparing guluronic acid oligosacchride through Fenton's reagent comprises preparing wt. percentage concentration of 0.1-5% guluronic polyuronic acid aqueous solution, characterized in that charging oxidizing agent into the aqueous solution till the concentration is between 0.05-0.5%, charging metallic ions to concentration of 0.05-0.5%, reacting at the temperature of 20-40 deg. C, after the reaction ends, condensing the reaction liquid, carrying out separation and purification through Bio-gel P10, Q-Sepharose Fast Flow chromatography columns. The process for preparing guluronic acid oligosacchride according to the invention features fast reaction rate and high product purity. 一种利用芬顿反应制备古罗糖醛酸寡糖的方法，包括将聚古罗糖醛酸配成重量百分浓度为0.1～5％的水溶液，其特征是向该水溶液加入氧化剂至浓度为0.05-0.5％，加入金属离子至浓度0.05-0.5％，在20～40℃下反应，反应中止后，将反应液浓缩，然后用Bio-gelP10，Q-Sepharose Fast Flow层析柱分离纯化。本发明的制备古罗糖醛酸寡糖的方法，反应速度快，产物纯度高，易于分离纯化，能大量生产古罗糖醛酸寡糖，使其得到充分利用，其功能和活性也能得到广泛的开发。