High accuracy rate biologic enzyme blood type diagnosis reagent and preparation method thereof

• Main Author: KAIQUAN,PENG SONG
• Format: Patent
• Language: Chinese; English
• Published: 01.09.2004
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention relates to a high-accuracy blood type diagnosing reagent by blood type serological immune reaction, specially a blood type diagnosing reagent of Rh, Kidd and so on and cross-mixed blood as well as preparing method. The diagnosing reagent includes pawpaw proteinase powder and enzyme inhibitor, pawpaw proteinase diluted solution and enzyme inhibitor diluted solution, and the preparing method: firstly cultivate streptomycin by activated culture medium, then enlarge culture, and finally make industrialized production in fermentation cylinder, and then obtain the finished product through inactivation, purification, condensation and drying. Compared with other medium reagents, the diagnosing agent has characters of higher detecting sensitivity, accurate and reliable identification result, simple method, etc, applied in vitro blood type serological diagnosis tests such as antibody screening test, antibody identification test, antibody effect titration test, transfusion compatibility test, and sensitive blood type identification test. 本发明涉及一种血型血清学免疫反应高准确率血型诊断试剂，特别是Rh、Kidd等血型及交叉配血的诊断试剂及其制备方法，它包括木瓜蛋白酶粉和酶抑制剂、木瓜蛋白酶稀释液、酶抑制剂稀释液，其制备方法是先将链霉菌用活化培养基培养，再扩大培养，最后在发酵罐进行工业化生产，经灭活、纯化、浓缩、干燥得成品，该诊断试剂与其它介质类试剂相比，具有检测灵敏度较高，鉴定结果准确可靠，方法简单，血型及交叉配血诊断试剂结果真实可靠的特点，适用于体外血型血清学诊断实验中抗体筛检实验，抗体鉴定实验、抗体效价滴定实验、输血相容性实验、敏感型血型鉴定实验。