High expressed high temperature resistant phytase gene in methanol yeast

• Main Authors: AISHENG XIONG, QUANHONG YAO, RIHE PENG
• Format: Patent
• Language: Chinese; English
• Published: 18.02.2004
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

A refractory phytase gene with high expression in methanol yeast is prepared through PCR amplification and recombining its DNA, that is, configuring mutant phytase gene liabrary on prokaryotic expression plasmid, screening refractory phytase gene, changing its preferably codon to make it be expressed in yeast, and electric shock transfer to integrate it in Pichia yeast for high expression. 本发明提供了一种在甲醇酵母中高表达的耐高温植酸酶基因以及它的制备方法，本发明采用PCR扩增技术合成耐高温植酸酶基因，并对其进行DNA改组，在原核表达质粒上构建突变植酸酶基因文库，通过突变植酸酶筛选获得耐高温植酸酶基因，通过改变基因的偏爱密码，使基因适合在酵母中表达，通过电击转化将耐高温的植酸酶基因整合到毕赤酵母中实现高表达。