Technology of preparing rat nerve growth factor using organic solvent virus deactivation method

A process for preparing the rat nerve growth factor (NGF) by using organic solvent to deactivate virus includes such steps as taking the submaxillary gland tissue of rat, homogenizing, obtaining supernatant, centrifugal deposition, removing deposit, ion-exchange chromatography, acidifying for dissoc...

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Bibliographic Details
Main Author GUOCHENG,TANG YANG
Format Patent
LanguageEnglish
Published 28.02.2007
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Summary:A process for preparing the rat nerve growth factor (NGF) by using organic solvent to deactivate virus includes such steps as taking the submaxillary gland tissue of rat, homogenizing, obtaining supernatant, centrifugal deposition, removing deposit, ion-exchange chromatography, acidifying for dissociating, centrifugal separation to obtain supernatant, deluting by the water for injection, adding organic and detergent, ion-exchange chromatography, collecting target protein peak, ion-exchange chromatography to obtain raw NGF liquid, adding mannitol and albumin, filter and freeze drying.
Bibliography:Application Number: CN200510019392