Specific DNA sequence of oriental vole and its use

The present invention discloses one specific DNA sequence of oriental vole, which is one Japanese blood fluke resisting related gene sequence. Through designing primer based on mouse H2 extron 2 and its two-side sequence to amplity oriental vole genome DNA, measuring sequence of amplified product an...

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Bibliographic Details
Main Authors XINGHUA PENG, WEIXIN HU, RONG YANG
Format Patent
LanguageEnglish
Published 16.05.2001
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
SPIRITS
VINEGAR
WINE
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Summary:The present invention discloses one specific DNA sequence of oriental vole, which is one Japanese blood fluke resisting related gene sequence. Through designing primer based on mouse H2 extron 2 and its two-side sequence to amplity oriental vole genome DNA, measuring sequence of amplified product and designing and synthesizing inner primer to perform in vitro amplification of oriental vole genomeDNA, specific amplification band is obtained. The sequence analysis shows that Japanese blood fluke resisting related gene sequence exists only in oriental vole genome and has one polypeptide chain-encoding extron. The related gene sequence may be utilized in gene sorting, species identification and determining molecular genetics mark, and has important application value in preparing gene medicine resisting Japanese blood fluke.
Bibliography:Application Number: CN20001026606