HSV-1 virus carrier system, method for building-up and quick guiding-in therewith

The present invention relates to a HSV-1 virus carrier system and its constitution and quick introduction method. It is characterized by cloning the complementary DNA (cDNA) functional fragment of exogenous target gene NGF gene onto the enzymic incision site of incision enzyme BamH1 of virus carrier...

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Bibliographic Details
Main Authors CHENGGUANG SUI, CHAODONG ZHANG, CHANGSHAN REN
Format Patent
LanguageEnglish
Published 26.01.2000
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention relates to a HSV-1 virus carrier system and its constitution and quick introduction method. It is characterized by cloning the complementary DNA (cDNA) functional fragment of exogenous target gene NGF gene onto the enzymic incision site of incision enzyme BamH1 of virus carrier, tranfecting the constituted plasmid and packaging so as to constitute the invented HSV-1 virus carrier system. By utilizing the characteristics of that the virus possesses the ecotropic property for nerve, the olfactory nerve is selected and used as introduction channel of said virus carrier, then the obtained high-titer HSV-1 virus carrier 0.1 ml (concentration 16 to the power 6 ptu/ml) is injected from patient's nasal cavity, and said carrier can be quickly come into the pathologic changedposition of Alzheimer's disease so as to attain the goal of gene therapy of the Alzheimer's disease.
Bibliography:Application Number: CN1998114171