一种利用细菌来源聚集诱导发光剂识别细胞内外G-四链体的方法

本申请涉及一种利用G4荧光探针2-(2-羟基-6-甲氧基-3-丙酰基苯基)喹唑啉-4(3H)-酮(HMPQ)识别细胞中G-四链体(G4)的方法。该探针对G-四链体具有特异性结合亲和力，能够在不改变其构象的情况下照亮这些非规范核酸结构。G4参与染色质组织、基因调控和基因组稳定性，并似乎有助于癌症的生长和进展。通过利用HMPQ的独特荧光机制(即，激发态分子内质子转移)，该方法能够实现对细胞核中G4结构的无标记精确检测。本申请还涉及一种用于选择性检测细胞中G-四链体(G4)的无标记试剂盒。 The subject invention pertains to a method for identify...

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Bibliographic Details
Format Patent
LanguageChinese
Published 08.08.2025
Subjects
ADHESIVES
CHEMISTRY
DYES
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE
MEASURING
METALLURGY
MISCELLANEOUS APPLICATIONS OF MATERIALS
MISCELLANEOUS COMPOSITIONS
NATURAL RESINS
PAINTS
PHYSICS
POLISHES
TESTING
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Summary:本申请涉及一种利用G4荧光探针2-(2-羟基-6-甲氧基-3-丙酰基苯基)喹唑啉-4(3H)-酮(HMPQ)识别细胞中G-四链体(G4)的方法。该探针对G-四链体具有特异性结合亲和力，能够在不改变其构象的情况下照亮这些非规范核酸结构。G4参与染色质组织、基因调控和基因组稳定性，并似乎有助于癌症的生长和进展。通过利用HMPQ的独特荧光机制(即，激发态分子内质子转移)，该方法能够实现对细胞核中G4结构的无标记精确检测。本申请还涉及一种用于选择性检测细胞中G-四链体(G4)的无标记试剂盒。 The subject invention pertains to a method for identifying G-quadruplexes (G4s) in a cell using a G4 fluorescent probe, 2-(2-hydroxy-6-methoxy-3-propionylphenyl)quinazolin-4(3H)-one (HMPQ), which exhibits specific binding affinity to G-quadruplexes (G4s), illuminating these non-canonical nucleic acid structures without altering their conformation. G4s are involved in chromatin organisation, gene regulation, genome stability, and appear to contribute to cancer growth and progression. By utilizing HMPQ's unique fluorescence mechanism through excited-state intramolecular proton transfer, the method allows label-free precise detection of G4 structures in the nucleus of a cell.
Bibliography:Application Number: CN202510133608