Colorimetric biosensor and application thereof in colorimetric detection of campylobacter jejuni

• Main Authors: LIU JINQIU, DU QINGBAO, LIU WEIXING, LIU CHENGUANG, LI DONG, ZHONG YUANXIA, GE ANSHAN, WANG JIAOKUN
• Format: Patent
• Language: Chinese; English
• Published: 04.04.2025
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention belongs to the technical field of biological detection, and relates to a campylobacter jejuni detection method, in particular to a colorimetric biosensor and application thereof in detecting campylobacter jejuni through a colorimetric method. The colorimetric biosensor comprises: a capture module comprising magnetic nanoparticles and an aptamer modified on the magnetic nanoparticles, the aptamer being capable of specifically binding with campylobacter jejuni; the binding-conduction module is used for binding campylobacter jejuni and comprises a siderophore and polylysine modified on the surface of the siderophore; the color developing module is used for preparing a gold nanoparticle solution. The colorimetric biosensor provided by the invention can be used for detecting campylobacter jejuni, and has the advantages of high sensitivity, strong specificity, good stability, accurate quantification and the like. 本发明属于生物检测技术领域，涉及空肠弯曲菌的检测方法，具体涉及一种比色生物传感器及其在比色法检测空肠弯曲菌中的应用。比色生物传感器包括：捕获模块，包括磁性纳米颗粒和修饰在所述磁性