Multi-vector recombinase mediated cassette exchange

• Main Authors: SHEN ALEX, BARNARD, GEORGE, C, NG CHIU KIN DAMON
• Format: Patent
• Language: Chinese; English
• Published: 28.03.2025
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The presently disclosed subject matter relates to multi-vector recombinase-mediated cassette exchange methods to achieve targeted integration of sequences of interest for producing host cells that express recombinant proteins such as monoclonal antibodies, as well as components derived therefrom such as bispecific antibodies, such as bispecific antibodies. And other complex forms of proteins such as membrane protein complexes, and other molecules that are difficult to express. 当前公开的主题涉及多载体重组酶介导的盒式交换方法，以实现针对生成宿主细胞的目标序列的靶向整合，所述生成宿主细胞表达重组蛋白例如单克隆抗体，以及由其衍生的组分例如双特异性抗体，及其他复杂形式蛋白质例如膜蛋白复合物，以及其他难以表达的分子。