Method for culturing low-temperature-resistant variety of pinctada martensii by using LncRNA as molecular marker and application

• Main Authors: LIANG MINXIN, WANG PING, LIAO YONGSHAN, REN ZIQI, YU WENHAO, JEONG CHEOL, WANG QINGHENG, CHEN LINHONG, HUANG YIWEI
• Format: Patent
• Language: Chinese; English
• Published: 14.03.2025
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for culturing a low-temperature-resistant variety of Pinctada martensii by using LncRNA as a molecular marker and application of the method, and relates to the technical field of aquatic culture, and the key points of the technical scheme are as follows: the method comprises the following steps: extracting genome DNA from the low-temperature-resistant Pinctada martensii strain; amplifying a DNA (deoxyribonucleic acid) fragment containing the lnclont allele by using a PCR (polymerase chain reaction) technology; then detecting the amplified DNA fragment by using a specific molecular marker technology, and determining whether an lnrun allele exists or not; carrying out statistical analysis on data obtained by molecular marker analysis, and judging the low-temperature tolerance of the pinctada martensii individuals; and finally, screening out low-temperature-resistant pinctada martensii individuals according to a data processing result, and performing biological verification to en