Method for detecting non-endotoxin components in bacterial endotoxin standard substance

• Main Authors: TAN CHAOHONG, ZHANG CAN, BAI MIAO, ZHANG MINGLU
• Format: Patent
• Language: Chinese; English
• Published: 30.08.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention provides a method for detecting non-endotoxin components in bacterial endotoxin standard substances, which comprises the following steps of: identifying non-endotoxin component excipients and biological impurities in different bacterial endotoxin standard substances by respectively adopting an ultraviolet absorption spectrum and a three-dimensional fluorescence spectrum, and further confirming biological pollution components through a cell TLRs test. In addition, the inflammation induction capacities of different bacterial endotoxin standard substances are compared by adopting a mononuclear cell activation reaction determination method. The method is beneficial to detection and identification of non-endotoxin components in the bacterial endotoxin standard substance, and effective quality control of bacterial endotoxin related detection methods such as tachypleus tridentatus test and mononuclear cell activation reaction determination is realized. 本发明提供一种细菌内毒素标准物质中非内毒素成分的检测方法，本发明分别采用紫外吸收光谱和三维荧光光谱识